antibody against uea1 Search Results


uea 1  (Vector Laboratories)
96
Vector Laboratories uea 1
(A) Thymi were isolated from mice of the indicated genotypes (32–45 days old) and processed for H&E staining. Sections are shown at different magnifications to illustrate the severe thymic aplasia in the Foxn1933/933 and Foxn1933/1089 genotypes. Scale bars: 1 mm. (B) IHC was performed on thymic tissue sections with antibodies detecting cTECs (cytokeratin 8) and mTECs (cytokeratin 5). Two independent tissue samples were used per genotype and were labeled as the first set and second set. Scale bars: 0.5 mm. (C) Dispersed thymic tissues were prepared from mice of the indicated genotypes, and TECs were compared by flow cytometry, first by gating on EpCAM+CD45– cells with antibodies detecting cTECs (BP-1) and mTECs <t>(UEA-1)</t> along with the cell-surface levels of MHC CII. (D and E) mTEC populations (UEA-1) were costained with antibodies detecting MHC CII in mice of the indicated genotypes, and dot blot comparisons are shown. (E) The MHC CII expression was compared between control littermates (black line) and Foxn11089/1089 mice (gray line). (F) The percentage of the different cTEC and mTEC subsets in the thymus of the indicated mice was calculated (n = 4 mice/group). Statistical analysis was performed using a Student’s t test.
Uea 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uea 1/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
uea 1 - by Bioz Stars, 2026-03
96/100 stars
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antibodies against cd3  (Thermo Fisher)
90
Thermo Fisher antibodies against cd3
(A) Thymi were isolated from mice of the indicated genotypes (32–45 days old) and processed for H&E staining. Sections are shown at different magnifications to illustrate the severe thymic aplasia in the Foxn1933/933 and Foxn1933/1089 genotypes. Scale bars: 1 mm. (B) IHC was performed on thymic tissue sections with antibodies detecting cTECs (cytokeratin 8) and mTECs (cytokeratin 5). Two independent tissue samples were used per genotype and were labeled as the first set and second set. Scale bars: 0.5 mm. (C) Dispersed thymic tissues were prepared from mice of the indicated genotypes, and TECs were compared by flow cytometry, first by gating on EpCAM+CD45– cells with antibodies detecting cTECs (BP-1) and mTECs <t>(UEA-1)</t> along with the cell-surface levels of MHC CII. (D and E) mTEC populations (UEA-1) were costained with antibodies detecting MHC CII in mice of the indicated genotypes, and dot blot comparisons are shown. (E) The MHC CII expression was compared between control littermates (black line) and Foxn11089/1089 mice (gray line). (F) The percentage of the different cTEC and mTEC subsets in the thymus of the indicated mice was calculated (n = 4 mice/group). Statistical analysis was performed using a Student’s t test.
Antibodies Against Cd3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against cd3/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
antibodies against cd3 - by Bioz Stars, 2026-03
90/100 stars
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ulex europaeus agglutinin-1 (uea-1  (Millipore)
90
Millipore ulex europaeus agglutinin-1 (uea-1
(A) Thymi were isolated from mice of the indicated genotypes (32–45 days old) and processed for H&E staining. Sections are shown at different magnifications to illustrate the severe thymic aplasia in the Foxn1933/933 and Foxn1933/1089 genotypes. Scale bars: 1 mm. (B) IHC was performed on thymic tissue sections with antibodies detecting cTECs (cytokeratin 8) and mTECs (cytokeratin 5). Two independent tissue samples were used per genotype and were labeled as the first set and second set. Scale bars: 0.5 mm. (C) Dispersed thymic tissues were prepared from mice of the indicated genotypes, and TECs were compared by flow cytometry, first by gating on EpCAM+CD45– cells with antibodies detecting cTECs (BP-1) and mTECs <t>(UEA-1)</t> along with the cell-surface levels of MHC CII. (D and E) mTEC populations (UEA-1) were costained with antibodies detecting MHC CII in mice of the indicated genotypes, and dot blot comparisons are shown. (E) The MHC CII expression was compared between control littermates (black line) and Foxn11089/1089 mice (gray line). (F) The percentage of the different cTEC and mTEC subsets in the thymus of the indicated mice was calculated (n = 4 mice/group). Statistical analysis was performed using a Student’s t test.
Ulex Europaeus Agglutinin 1 (Uea 1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ulex europaeus agglutinin-1 (uea-1/product/Millipore
Average 90 stars, based on 1 article reviews
ulex europaeus agglutinin-1 (uea-1 - by Bioz Stars, 2026-03
90/100 stars
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antibodies against endothelial cell-specific lectin ulex europaeus agglutinin-1 (uea-1  (Millipore)
90
Millipore antibodies against endothelial cell-specific lectin ulex europaeus agglutinin-1 (uea-1
(A) Thymi were isolated from mice of the indicated genotypes (32–45 days old) and processed for H&E staining. Sections are shown at different magnifications to illustrate the severe thymic aplasia in the Foxn1933/933 and Foxn1933/1089 genotypes. Scale bars: 1 mm. (B) IHC was performed on thymic tissue sections with antibodies detecting cTECs (cytokeratin 8) and mTECs (cytokeratin 5). Two independent tissue samples were used per genotype and were labeled as the first set and second set. Scale bars: 0.5 mm. (C) Dispersed thymic tissues were prepared from mice of the indicated genotypes, and TECs were compared by flow cytometry, first by gating on EpCAM+CD45– cells with antibodies detecting cTECs (BP-1) and mTECs <t>(UEA-1)</t> along with the cell-surface levels of MHC CII. (D and E) mTEC populations (UEA-1) were costained with antibodies detecting MHC CII in mice of the indicated genotypes, and dot blot comparisons are shown. (E) The MHC CII expression was compared between control littermates (black line) and Foxn11089/1089 mice (gray line). (F) The percentage of the different cTEC and mTEC subsets in the thymus of the indicated mice was calculated (n = 4 mice/group). Statistical analysis was performed using a Student’s t test.
Antibodies Against Endothelial Cell Specific Lectin Ulex Europaeus Agglutinin 1 (Uea 1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against endothelial cell-specific lectin ulex europaeus agglutinin-1 (uea-1/product/Millipore
Average 90 stars, based on 1 article reviews
antibodies against endothelial cell-specific lectin ulex europaeus agglutinin-1 (uea-1 - by Bioz Stars, 2026-03
90/100 stars
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ec-specific antibody en4  (Chemie GmbH)
90
Chemie GmbH ec-specific antibody en4
(A) Thymi were isolated from mice of the indicated genotypes (32–45 days old) and processed for H&E staining. Sections are shown at different magnifications to illustrate the severe thymic aplasia in the Foxn1933/933 and Foxn1933/1089 genotypes. Scale bars: 1 mm. (B) IHC was performed on thymic tissue sections with antibodies detecting cTECs (cytokeratin 8) and mTECs (cytokeratin 5). Two independent tissue samples were used per genotype and were labeled as the first set and second set. Scale bars: 0.5 mm. (C) Dispersed thymic tissues were prepared from mice of the indicated genotypes, and TECs were compared by flow cytometry, first by gating on EpCAM+CD45– cells with antibodies detecting cTECs (BP-1) and mTECs <t>(UEA-1)</t> along with the cell-surface levels of MHC CII. (D and E) mTEC populations (UEA-1) were costained with antibodies detecting MHC CII in mice of the indicated genotypes, and dot blot comparisons are shown. (E) The MHC CII expression was compared between control littermates (black line) and Foxn11089/1089 mice (gray line). (F) The percentage of the different cTEC and mTEC subsets in the thymus of the indicated mice was calculated (n = 4 mice/group). Statistical analysis was performed using a Student’s t test.
Ec Specific Antibody En4, supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ec-specific antibody en4/product/Chemie GmbH
Average 90 stars, based on 1 article reviews
ec-specific antibody en4 - by Bioz Stars, 2026-03
90/100 stars
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epcam  (Thermo Fisher)
86
Thermo Fisher epcam
(A) Thymi were isolated from mice of the indicated genotypes (32–45 days old) and processed for H&E staining. Sections are shown at different magnifications to illustrate the severe thymic aplasia in the Foxn1933/933 and Foxn1933/1089 genotypes. Scale bars: 1 mm. (B) IHC was performed on thymic tissue sections with antibodies detecting cTECs (cytokeratin 8) and mTECs (cytokeratin 5). Two independent tissue samples were used per genotype and were labeled as the first set and second set. Scale bars: 0.5 mm. (C) Dispersed thymic tissues were prepared from mice of the indicated genotypes, and TECs were compared by flow cytometry, first by gating on EpCAM+CD45– cells with antibodies detecting cTECs (BP-1) and mTECs <t>(UEA-1)</t> along with the cell-surface levels of MHC CII. (D and E) mTEC populations (UEA-1) were costained with antibodies detecting MHC CII in mice of the indicated genotypes, and dot blot comparisons are shown. (E) The MHC CII expression was compared between control littermates (black line) and Foxn11089/1089 mice (gray line). (F) The percentage of the different cTEC and mTEC subsets in the thymus of the indicated mice was calculated (n = 4 mice/group). Statistical analysis was performed using a Student’s t test.
Epcam, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/epcam/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
epcam - by Bioz Stars, 2026-03
86/100 stars
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uea-1  (Reactolab sa)
90
Reactolab sa uea-1
(A) Thymi were isolated from mice of the indicated genotypes (32–45 days old) and processed for H&E staining. Sections are shown at different magnifications to illustrate the severe thymic aplasia in the Foxn1933/933 and Foxn1933/1089 genotypes. Scale bars: 1 mm. (B) IHC was performed on thymic tissue sections with antibodies detecting cTECs (cytokeratin 8) and mTECs (cytokeratin 5). Two independent tissue samples were used per genotype and were labeled as the first set and second set. Scale bars: 0.5 mm. (C) Dispersed thymic tissues were prepared from mice of the indicated genotypes, and TECs were compared by flow cytometry, first by gating on EpCAM+CD45– cells with antibodies detecting cTECs (BP-1) and mTECs <t>(UEA-1)</t> along with the cell-surface levels of MHC CII. (D and E) mTEC populations (UEA-1) were costained with antibodies detecting MHC CII in mice of the indicated genotypes, and dot blot comparisons are shown. (E) The MHC CII expression was compared between control littermates (black line) and Foxn11089/1089 mice (gray line). (F) The percentage of the different cTEC and mTEC subsets in the thymus of the indicated mice was calculated (n = 4 mice/group). Statistical analysis was performed using a Student’s t test.
Uea 1, supplied by Reactolab sa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uea-1/product/Reactolab sa
Average 90 stars, based on 1 article reviews
uea-1 - by Bioz Stars, 2026-03
90/100 stars
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mhc ii (i-a/i-e  (Cytek Biosciences)
90
Cytek Biosciences mhc ii (i-a/i-e
(A) Thymi were isolated from mice of the indicated genotypes (32–45 days old) and processed for H&E staining. Sections are shown at different magnifications to illustrate the severe thymic aplasia in the Foxn1933/933 and Foxn1933/1089 genotypes. Scale bars: 1 mm. (B) IHC was performed on thymic tissue sections with antibodies detecting cTECs (cytokeratin 8) and mTECs (cytokeratin 5). Two independent tissue samples were used per genotype and were labeled as the first set and second set. Scale bars: 0.5 mm. (C) Dispersed thymic tissues were prepared from mice of the indicated genotypes, and TECs were compared by flow cytometry, first by gating on EpCAM+CD45– cells with antibodies detecting cTECs (BP-1) and mTECs <t>(UEA-1)</t> along with the cell-surface levels of MHC CII. (D and E) mTEC populations (UEA-1) were costained with antibodies detecting MHC CII in mice of the indicated genotypes, and dot blot comparisons are shown. (E) The MHC CII expression was compared between control littermates (black line) and Foxn11089/1089 mice (gray line). (F) The percentage of the different cTEC and mTEC subsets in the thymus of the indicated mice was calculated (n = 4 mice/group). Statistical analysis was performed using a Student’s t test.
Mhc Ii (I A/I E, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mhc ii (i-a/i-e/product/Cytek Biosciences
Average 90 stars, based on 1 article reviews
mhc ii (i-a/i-e - by Bioz Stars, 2026-03
90/100 stars
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antibodies against cd45 (30-f11  (Becton Dickinson)
90
Becton Dickinson antibodies against cd45 (30-f11
TEC shift in E-ARKO mice. (A) Gating strategy for TECs. (B–D) Relative numbers of all thymic epithelial cells (TECs), cortical (cTEC; <t>CD45</t> − EpCAM + UEA1 − Ly51 + ), and medullary (mTEC; CD45 − EpCAM + UEA1 + Ly51 − ) TEC in control and E-ARKO male mice; n = 8/group. (E) Assessment of AR knockout by measurement of exon 2 gDNA in mTECs from 4-week-old control (K5-Cre + ; n = 4) and E-ARKO (AR fl K5-Cre + ; n = 4) male mice. (F) Assessment of AR knockout by measurement of exon 2 gDNA in cTECs from 8-week-old control (K5-Cre + ; n = 4 pools, 2 mice/pool) and E-ARKO (AR fl K5-Cre + ; n = 6 pools, 2 mice/pool) male mice. * P < 0.05 (Mann-Whitney U test); all bars indicate means; circles represent individual mice, error bars indicate SEM.
Antibodies Against Cd45 (30 F11, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against cd45 (30-f11/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
antibodies against cd45 (30-f11 - by Bioz Stars, 2026-03
90/100 stars
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ly51 (6c3  (Becton Dickinson)
90
Becton Dickinson ly51 (6c3
TEC shift in E-ARKO mice. (A) Gating strategy for TECs. (B–D) Relative numbers of all thymic epithelial cells (TECs), cortical (cTEC; CD45 − EpCAM + UEA1 − <t>Ly51</t> + ), and medullary (mTEC; CD45 − EpCAM + UEA1 + Ly51 − ) TEC in control and E-ARKO male mice; n = 8/group. (E) Assessment of AR knockout by measurement of exon 2 gDNA in mTECs from 4-week-old control (K5-Cre + ; n = 4) and E-ARKO (AR fl K5-Cre + ; n = 4) male mice. (F) Assessment of AR knockout by measurement of exon 2 gDNA in cTECs from 8-week-old control (K5-Cre + ; n = 4 pools, 2 mice/pool) and E-ARKO (AR fl K5-Cre + ; n = 6 pools, 2 mice/pool) male mice. * P < 0.05 (Mann-Whitney U test); all bars indicate means; circles represent individual mice, error bars indicate SEM.
Ly51 (6c3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ly51 (6c3/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
ly51 (6c3 - by Bioz Stars, 2026-03
90/100 stars
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biotinylated ulex europaeus agglutinin 1 uea 1  (Vector Laboratories)
96
Vector Laboratories biotinylated ulex europaeus agglutinin 1 uea 1
TEC shift in E-ARKO mice. (A) Gating strategy for TECs. (B–D) Relative numbers of all thymic epithelial cells (TECs), cortical (cTEC; CD45 − EpCAM + UEA1 − <t>Ly51</t> + ), and medullary (mTEC; CD45 − EpCAM + UEA1 + Ly51 − ) TEC in control and E-ARKO male mice; n = 8/group. (E) Assessment of AR knockout by measurement of exon 2 gDNA in mTECs from 4-week-old control (K5-Cre + ; n = 4) and E-ARKO (AR fl K5-Cre + ; n = 4) male mice. (F) Assessment of AR knockout by measurement of exon 2 gDNA in cTECs from 8-week-old control (K5-Cre + ; n = 4 pools, 2 mice/pool) and E-ARKO (AR fl K5-Cre + ; n = 6 pools, 2 mice/pool) male mice. * P < 0.05 (Mann-Whitney U test); all bars indicate means; circles represent individual mice, error bars indicate SEM.
Biotinylated Ulex Europaeus Agglutinin 1 Uea 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated ulex europaeus agglutinin 1 uea 1/product/Vector Laboratories
Average 96 stars, based on 1 article reviews
biotinylated ulex europaeus agglutinin 1 uea 1 - by Bioz Stars, 2026-03
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Image Search Results


(A) Thymi were isolated from mice of the indicated genotypes (32–45 days old) and processed for H&E staining. Sections are shown at different magnifications to illustrate the severe thymic aplasia in the Foxn1933/933 and Foxn1933/1089 genotypes. Scale bars: 1 mm. (B) IHC was performed on thymic tissue sections with antibodies detecting cTECs (cytokeratin 8) and mTECs (cytokeratin 5). Two independent tissue samples were used per genotype and were labeled as the first set and second set. Scale bars: 0.5 mm. (C) Dispersed thymic tissues were prepared from mice of the indicated genotypes, and TECs were compared by flow cytometry, first by gating on EpCAM+CD45– cells with antibodies detecting cTECs (BP-1) and mTECs (UEA-1) along with the cell-surface levels of MHC CII. (D and E) mTEC populations (UEA-1) were costained with antibodies detecting MHC CII in mice of the indicated genotypes, and dot blot comparisons are shown. (E) The MHC CII expression was compared between control littermates (black line) and Foxn11089/1089 mice (gray line). (F) The percentage of the different cTEC and mTEC subsets in the thymus of the indicated mice was calculated (n = 4 mice/group). Statistical analysis was performed using a Student’s t test.

Journal: The Journal of Clinical Investigation

Article Title: FOXN1 compound heterozygous mutations cause selective thymic hypoplasia in humans

doi: 10.1172/JCI127565

Figure Lengend Snippet: (A) Thymi were isolated from mice of the indicated genotypes (32–45 days old) and processed for H&E staining. Sections are shown at different magnifications to illustrate the severe thymic aplasia in the Foxn1933/933 and Foxn1933/1089 genotypes. Scale bars: 1 mm. (B) IHC was performed on thymic tissue sections with antibodies detecting cTECs (cytokeratin 8) and mTECs (cytokeratin 5). Two independent tissue samples were used per genotype and were labeled as the first set and second set. Scale bars: 0.5 mm. (C) Dispersed thymic tissues were prepared from mice of the indicated genotypes, and TECs were compared by flow cytometry, first by gating on EpCAM+CD45– cells with antibodies detecting cTECs (BP-1) and mTECs (UEA-1) along with the cell-surface levels of MHC CII. (D and E) mTEC populations (UEA-1) were costained with antibodies detecting MHC CII in mice of the indicated genotypes, and dot blot comparisons are shown. (E) The MHC CII expression was compared between control littermates (black line) and Foxn11089/1089 mice (gray line). (F) The percentage of the different cTEC and mTEC subsets in the thymus of the indicated mice was calculated (n = 4 mice/group). Statistical analysis was performed using a Student’s t test.

Article Snippet: The cells were stained with antibodies against CD45 (Tonbo Scientific), MHC CII (I-A/I-E) (Tonbo Scientific), EpCAM (eBioscience), BP-1 (eBioscience), and UEA-1 (Vector Laboratories).

Techniques: Isolation, Staining, Labeling, Flow Cytometry, Dot Blot, Expressing

TEC shift in E-ARKO mice. (A) Gating strategy for TECs. (B–D) Relative numbers of all thymic epithelial cells (TECs), cortical (cTEC; CD45 − EpCAM + UEA1 − Ly51 + ), and medullary (mTEC; CD45 − EpCAM + UEA1 + Ly51 − ) TEC in control and E-ARKO male mice; n = 8/group. (E) Assessment of AR knockout by measurement of exon 2 gDNA in mTECs from 4-week-old control (K5-Cre + ; n = 4) and E-ARKO (AR fl K5-Cre + ; n = 4) male mice. (F) Assessment of AR knockout by measurement of exon 2 gDNA in cTECs from 8-week-old control (K5-Cre + ; n = 4 pools, 2 mice/pool) and E-ARKO (AR fl K5-Cre + ; n = 6 pools, 2 mice/pool) male mice. * P < 0.05 (Mann-Whitney U test); all bars indicate means; circles represent individual mice, error bars indicate SEM.

Journal: Frontiers in Immunology

Article Title: Androgen Receptors in Epithelial Cells Regulate Thymopoiesis and Recent Thymic Emigrants in Male Mice

doi: 10.3389/fimmu.2020.01342

Figure Lengend Snippet: TEC shift in E-ARKO mice. (A) Gating strategy for TECs. (B–D) Relative numbers of all thymic epithelial cells (TECs), cortical (cTEC; CD45 − EpCAM + UEA1 − Ly51 + ), and medullary (mTEC; CD45 − EpCAM + UEA1 + Ly51 − ) TEC in control and E-ARKO male mice; n = 8/group. (E) Assessment of AR knockout by measurement of exon 2 gDNA in mTECs from 4-week-old control (K5-Cre + ; n = 4) and E-ARKO (AR fl K5-Cre + ; n = 4) male mice. (F) Assessment of AR knockout by measurement of exon 2 gDNA in cTECs from 8-week-old control (K5-Cre + ; n = 4 pools, 2 mice/pool) and E-ARKO (AR fl K5-Cre + ; n = 6 pools, 2 mice/pool) male mice. * P < 0.05 (Mann-Whitney U test); all bars indicate means; circles represent individual mice, error bars indicate SEM.

Article Snippet: After incubation with FcR block (CD16/CD32, BD Biosciences), antibodies against CD45 (30-F11, BD Biosciences) and EpCAM/CD326 (G8.8, BD Biosciences), Ly51 (6C3, BD Biosciences) and the biotinylated lectin UEA-1 (Vector Laboratories) were added.

Techniques: Knock-Out, MANN-WHITNEY

TEC shift in E-ARKO mice. (A) Gating strategy for TECs. (B–D) Relative numbers of all thymic epithelial cells (TECs), cortical (cTEC; CD45 − EpCAM + UEA1 − Ly51 + ), and medullary (mTEC; CD45 − EpCAM + UEA1 + Ly51 − ) TEC in control and E-ARKO male mice; n = 8/group. (E) Assessment of AR knockout by measurement of exon 2 gDNA in mTECs from 4-week-old control (K5-Cre + ; n = 4) and E-ARKO (AR fl K5-Cre + ; n = 4) male mice. (F) Assessment of AR knockout by measurement of exon 2 gDNA in cTECs from 8-week-old control (K5-Cre + ; n = 4 pools, 2 mice/pool) and E-ARKO (AR fl K5-Cre + ; n = 6 pools, 2 mice/pool) male mice. * P < 0.05 (Mann-Whitney U test); all bars indicate means; circles represent individual mice, error bars indicate SEM.

Journal: Frontiers in Immunology

Article Title: Androgen Receptors in Epithelial Cells Regulate Thymopoiesis and Recent Thymic Emigrants in Male Mice

doi: 10.3389/fimmu.2020.01342

Figure Lengend Snippet: TEC shift in E-ARKO mice. (A) Gating strategy for TECs. (B–D) Relative numbers of all thymic epithelial cells (TECs), cortical (cTEC; CD45 − EpCAM + UEA1 − Ly51 + ), and medullary (mTEC; CD45 − EpCAM + UEA1 + Ly51 − ) TEC in control and E-ARKO male mice; n = 8/group. (E) Assessment of AR knockout by measurement of exon 2 gDNA in mTECs from 4-week-old control (K5-Cre + ; n = 4) and E-ARKO (AR fl K5-Cre + ; n = 4) male mice. (F) Assessment of AR knockout by measurement of exon 2 gDNA in cTECs from 8-week-old control (K5-Cre + ; n = 4 pools, 2 mice/pool) and E-ARKO (AR fl K5-Cre + ; n = 6 pools, 2 mice/pool) male mice. * P < 0.05 (Mann-Whitney U test); all bars indicate means; circles represent individual mice, error bars indicate SEM.

Article Snippet: After incubation with FcR block (CD16/CD32, BD Biosciences), antibodies against CD45 (30-F11, BD Biosciences) and EpCAM/CD326 (G8.8, BD Biosciences), Ly51 (6C3, BD Biosciences) and the biotinylated lectin UEA-1 (Vector Laboratories) were added.

Techniques: Knock-Out, MANN-WHITNEY